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KMID : 0613820220320080595
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Journal of Life Science
2022 Volume.32 No. 8 p.595 ~ p.600
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Analysis of Gene Diversity and Phenetic Relationship of Water Dropwort Species in Korea Using RAPD (OPB Primers) Markers
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Huh Man-Kyu
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Abstract
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Water dropworts, Oenanthe javanica and O. javanica var. japonica are called “minari” in Korea and are eaten as a vegetable. Cicuta virosa is common European water hemlock and has toxic properties, such as cicutoxin. Molecular variations of water dropwort species in Korea were investigated using random amplified polymorphic DNA (RAPD). The six populations were studied with 10 primers (Operon, OPB) for RAPD analysis. The 72 DNA fragments (bands) were found among six populations. Among these 72 bands, 61 (84.7%) bands were polymorphic. The typical populations of Cicuta virosa in Korea were small, isolated, and patchily distributed for natural populations and they maintained a high level of genetic diversity. However, when cultivated populations of O. javanica var. japonica were large and widely grown in rice paddies as vegetables, they maintained a lower genetic diversity than those of C. virosa and wild populations of O. javanica. Although the diversity indices of wild populations were shown to be higher than those of cultivated populations, no significant difference for measures of genetic variability was shown. Total genetic diversity value (HT) was 0.342. The interlocus variation in the within-population genetic diversity (HS) was 0.201. The proportion of total genetic variation due to differences among populations (GST) range was 0.414, indicating that 41.4% of the total variation was among populations. In conclusion, the RAPD technique was a useful method for discrimination between C. virosa and O. javanica. In addition, RAPD-OPB markers could further distinguish the strains from different food sources.
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KEYWORD
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Cicuta virosa, genetic diversity, Oenanthe javanica var. japonica, RAPD, water dropwort
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